GSBS Student Publications
GSBS Program
Neuroscience
UMMS Affiliation
Department of Neurobiology; Freeman Lab; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Date
2-19-2014
Document Type
Article
Medical Subject Headings
Animals; Chromatin Immunoprecipitation; Drosophila; Drosophila Proteins; Electrophysiological Processes; Homeodomain Proteins; Image Processing, Computer-Assisted; Immunohistochemistry; Microscopy, Confocal; Neuroglia; Neuromuscular Junction; RNA Interference; Real-Time Polymerase Chain Reaction; Receptors, Glutamate; Synapses; Transfection; Wnt Proteins
Disciplines
Developmental Neuroscience
Abstract
Glial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.
Rights and Permissions
Citation: J Neurosci. 2014 Feb 19;34(8):2910-20. doi: 10.1523/JNEUROSCI.3714-13.2014. Link to article on publisher's site
DOI of Published Version
10.1523/JNEUROSCI.3714-13.2014
Related Resources
Keywords
Drosophila, NMJ, glia, synapse, wnt/Wg
Journal Title
The Journal of neuroscience : the official journal of the Society for Neuroscience
PubMed ID
24553932
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Kerr, Kimberly S.; Fuentes Medel, Yuly F.; Brewer, Cassandra; Barria, Romina; Ashley, James A.; Abruzzi, Katharine C.; Sheehan, Amy E.; Tasdemir, Ozge E.; Freeman, Marc R.; and Budnik, Vivian, "Glial wingless/Wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction" (2014). GSBS Student Publications. 1921.
https://escholarship.umassmed.edu/gsbs_sp/1921
Comments
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Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license.