Department of Neurobiology; Freeman Lab; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Glial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.
Drosophila, NMJ, glia, synapse, wnt/Wg
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DOI of Published Version
J Neurosci. 2014 Feb 19;34(8):2910-20. doi: 10.1523/JNEUROSCI.3714-13.2014. Link to article on publisher's site
The Journal of neuroscience : the official journal of the Society for Neuroscience
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This work is licensed under a Creative Commons Attribution 4.0 License.
Kerr KS, Fuentes Medel YF, Brewer C, Barria R, Ashley JA, Abruzzi KC, Sheehan AE, Tasdemir OE, Freeman MR, Budnik V. (2014). Glial wingless/Wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1523/JNEUROSCI.3714-13.2014. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1921