GSBS Student Publications
Title
Construction and application of site-specific artificial nucleases for targeted gene editing
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Program in Gene Function and Expression
Date
1-2014
Document Type
Book Chapter
Medical Subject Headings
Animals; Animals, Genetically Modified; Base Sequence; DNA Primers; Deoxyribonucleases; Escherichia coli; Gene Targeting; *INDEL Mutation; Molecular Sequence Data; Protein Engineering; Substrate Specificity; Zebrafish; Zinc Fingers
Disciplines
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Genomics | Molecular Biology
Abstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Rights and Permissions
Citation: Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's site
DOI of Published Version
10.1007/978-1-62703-721-1_13
Related Resources
Journal Title
Methods in molecular biology (Clifton, N.J.)
PubMed ID
24233786
Repository Citation
Kok, Fatma O.; Gupta, Ankit; Lawson, Nathan D.; and Wolfe, Scot A., "Construction and application of site-specific artificial nucleases for targeted gene editing" (2014). GSBS Student Publications. 1912.
https://escholarship.umassmed.edu/gsbs_sp/1912