GSBS Student Publications
Title
Construction and application of site-specific artificial nucleases for targeted gene editing
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2014-1
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Program in Gene Function and Expression
Document Type
Book Chapter
Disciplines
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Genomics | Molecular Biology
Abstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
DOI of Published Version
10.1007/978-1-62703-721-1_13
Source
Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's site
Journal/Book/Conference Title
Methods in molecular biology (Clifton, N.J.)
Related Resources
PubMed ID
24233786
Repository Citation
Kok FO, Gupta A, Lawson ND, Wolfe SA. (2014). Construction and application of site-specific artificial nucleases for targeted gene editing. GSBS Student Publications. https://doi.org/10.1007/978-1-62703-721-1_13. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1912