SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases
Interdisciplinary Graduate Program
Program in Molecular Medicine
Biochemistry, Biophysics, and Structural Biology | Cellular and Molecular Physiology | Genetics and Genomics
The DNA damage response to double-strand breaks (DSBs) is critical for cellular viability. Recent work has shown that a host of chromatin regulators are recruited to a DSB, and that they are important for the DNA damage response. However, the functional relationships between different chromatin regulators at DSBs remain unclear. Here we describe a conserved functional interaction among the chromatin remodeling enzyme, SWI/SNF, the NuA4 and Gcn5 histone acetyltransferases, and phosphorylation of histone H2A.X (gammaH2AX). Specifically, we find that the NuA4 and Gcn5 enzymes are both required for the robust recruitment of SWI/SNF to a DSB, which in turn promotes the phosphorylation of H2A.X.
DOI of Published Version
DNA Repair (Amst). 2015 Jun;30:38-45. doi: 10.1016/j.dnarep.2015.03.006. Epub 2015 Mar 25. Link to article on publisher's site
Bennett GM, Peterson CL. (2015). SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1016/j.dnarep.2015.03.006. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1881