GSBS Student Publications
Title
Several rAAV vectors efficiently cross the blood-brain barrier and transduce neurons and astrocytes in the neonatal mouse central nervous system
GSBS Program
Interdisciplinary Graduate Program
UMMS Affiliation
Gene Therapy Center; Department of Microbiology and Physiology Systems; Department of Biochemistry and Molecular Pharmacology; Department of Pediatrics; Department of Neurology
Date
8-2011
Document Type
Article
Medical Subject Headings
Animals; Animals, Newborn; Astrocytes; Blood-Brain Barrier; Central Nervous System; Dependovirus; *Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Mice; Mice, Inbred C57BL; Neurons
Disciplines
Genetics | Genetics and Genomics | Molecular Genetics | Nervous System Diseases | Therapeutics
Abstract
Noninvasive systemic gene delivery to the central nervous system (CNS) has largely been impeded by the blood-brain barrier (BBB). Recent studies documented widespread CNS gene transfer after intravascular delivery of recombinant adeno-associated virus 9 (rAAV9). To investigate alternative and possibly more potent rAAV vectors for systemic gene delivery across the BBB, we systematically evaluated the CNS gene transfer properties of nine different rAAVEGFP vectors after intravascular infusion in neonatal mice. Several rAAVs efficiently transduce neurons, motor neurons, astrocytes, and Purkinje cells; among them, rAAVrh.10 is at least as efficient as rAAV9 in many of the regions examined. Importantly, intravenously delivered rAAVs did not cause abnormal microgliosis in the CNS. The rAAVs that achieve stable widespread gene transfer in the CNS are exceptionally useful platforms for the development of therapeutic approaches for neurological disorders affecting large regions of the CNS as well as convenient biological tools for neuroscience research.
Rights and Permissions
Citation: Mol Ther. 2011 Aug;19(8):1440-8. doi: 10.1038/mt.2011.98. Epub 2011 May 24. Link to article on publisher's site
DOI of Published Version
10.1038/mt.2011.98
Related Resources
Journal Title
Molecular therapy : the journal of the American Society of Gene Therapy
PubMed ID
21610699
Repository Citation
Zhang, Hongwei; Yang, Bin; Mu, Xin; Ahmed, Seemin Seher; Su, Qin; He, Ran; Wang, Hongyan; Mueller, Christian; Sena-Esteves, Miguel; Brown, Robert H. Jr.; Xu, Zuoshang; and Gao, Guangping, "Several rAAV vectors efficiently cross the blood-brain barrier and transduce neurons and astrocytes in the neonatal mouse central nervous system" (2011). GSBS Student Publications. 1879.
https://escholarship.umassmed.edu/gsbs_sp/1879