GSBS Student Publications

Title

Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence

Student Author(s)

Tingting Huang

GSBS Program

Cell Biology

Publication Date

2014-03-28

UMMS Affiliation

Department of Cell and Developmental Biology

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology | Developmental Biology

Abstract

Senescence-associated β-galactosidase (SA-β-gal) activity is widely used as a marker of cellular senescence and as an indicator of organismal aging. Here, we report that SA-β-gal activity is present in the visceral endoderm layer of early postimplantation mouse embryos in predictable patterns that vary as the embryo progresses in development. However, determination of the mitotic index and analysis of the expression of Cdkn1a (p21), a marker of senescent cells, do not indicate cellular senescence. Instead, analysis of embryos in culture revealed the presence of SA-β-gal activity in apical vacuoles of visceral endoderm cells likely a reflection of acidic β-galactosidase function in these organelles. SA-β-gal serves as a practical marker of the dynamics of the visceral endoderm that can be applied to developmental as well as functional studies of early mammalian embryos.

Keywords

SA-β-gal, apical vacuoles, embryo, mouse, yolk sac

DOI of Published Version

10.1002/dvg.22761

Source

Huang T, Rivera-Pérez JA. Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence. Genesis. 2014 Feb 25. doi: 10.1002/dvg.22761. Link to article on publisher's website

Journal/Book/Conference Title

Genesis

Related Resources

Link to article in PubMed

PubMed ID

24616249

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