Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence
Department of Cell and Developmental Biology
Cell Biology | Cellular and Molecular Physiology | Developmental Biology
Senescence-associated β-galactosidase (SA-β-gal) activity is widely used as a marker of cellular senescence and as an indicator of organismal aging. Here, we report that SA-β-gal activity is present in the visceral endoderm layer of early postimplantation mouse embryos in predictable patterns that vary as the embryo progresses in development. However, determination of the mitotic index and analysis of the expression of Cdkn1a (p21), a marker of senescent cells, do not indicate cellular senescence. Instead, analysis of embryos in culture revealed the presence of SA-β-gal activity in apical vacuoles of visceral endoderm cells likely a reflection of acidic β-galactosidase function in these organelles. SA-β-gal serves as a practical marker of the dynamics of the visceral endoderm that can be applied to developmental as well as functional studies of early mammalian embryos.
SA-β-gal, apical vacuoles, embryo, mouse, yolk sac
DOI of Published Version
Huang T, Rivera-Pérez JA. Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence. Genesis. 2014 Feb 25. doi: 10.1002/dvg.22761. Link to article on publisher's website
Huang T, Rivera-Perez JA. (2014). Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence. GSBS Student Publications. https://doi.org/10.1002/dvg.22761. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1853