Student Authors
Ryan T. HietpasUMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDepartment of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2011-05-10Keywords
Amino Acid Substitution; Codon; Computer Simulation; DNA, Fungal; Evolution, Molecular; Genes, Fungal; HSP90 Heat-Shock Proteins; Models, Genetic; Models, Molecular; Phylogeny; Point Mutation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Selection, GeneticEvolution
Molecular Biology
Molecular Genetics
Metadata
Show full item recordAbstract
The genes of all organisms have been shaped by selective pressures. The relationship between gene sequence and fitness has tremendous implications for understanding both evolutionary processes and functional constraints on the encoded proteins. Here, we have exploited deep sequencing technology to experimentally determine the fitness of all possible individual point mutants under controlled conditions for a nine-amino acid region of Hsp90. Over the past five decades, limited glimpses into the relationship between gene sequence and function have sparked a long debate regarding the distribution, relative proportion, and evolutionary significance of deleterious, neutral, and advantageous mutations. Our systematic experimental measurement of fitness effects of Hsp90 mutants in yeast, evaluated in the light of existing population genetic theory, are remarkably consistent with a nearly neutral model of molecular evolution.Source
Proc Natl Acad Sci U S A. 2011 May 10;108(19):7896-901. doi: 10.1073/pnas.1016024108. Link to article on publisher's site
DOI
10.1073/pnas.1016024108Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33308PubMed ID
21464309Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1073/pnas.1016024108