GSBS Student Publications

Title

Quaking regulates Hnrnpa1 expression through its 3' UTR in oligodendrocyte precursor cells

Student Author(s)

Brian Farley

GSBS Program

Biochemistry & Molecular Pharmacology

Publication Date

2011-01-06

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Document Type

Article

Disciplines

Biochemistry | Genetics and Genomics | Molecular Genetics

Abstract

In mice, Quaking (Qk) is required for myelin formation; in humans, it has been associated with psychiatric disease. QK regulates the stability, subcellular localization, and alternative splicing of several myelin-related transcripts, yet little is known about how QK governs these activities. Here, we show that QK enhances Hnrnpa1 mRNA stability by binding a conserved 3' UTR sequence with high affinity and specificity. A single nucleotide mutation in the binding site eliminates QK-dependent regulation, as does reduction of QK by RNAi. Analysis of exon expression across the transcriptome reveals that QK and hnRNP A1 regulate an overlapping subset of transcripts. Thus, a simple interpretation is that QK regulates a large set of oligodendrocyte precursor genes indirectly by increasing the intracellular concentration of hnRNP A1. Together, the data show that hnRNP A1 is an important QK target that contributes to its control of myelin gene expression.

DOI of Published Version

10.1371/journal.pgen.1001269

Source

PLoS Genet. 2011 Jan 6;7(1):e1001269. doi: 10.1371/journal.pgen.1001269. Link to article on publisher's site

Journal/Book/Conference Title

PLoS genetics

Related Resources

Link to Article in PubMed

PubMed ID

21253564

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