Amyloid precursor proteins anchor CPEB to membranes and promote polyadenylation-induced translation
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences
The cytoplasmic polyadenylation element (CPE) binding factor, CPEB, is a sequence-specific RNA binding protein that controls polyadenylation-induced translation in germ cells and at postsynaptic sites of neurons. A yeast two-hybrid screen with a mouse brain cDNA library identified the transmembrane amyloid precursor-like protein 1 (APLP1) as a CPEB-interacting factor. CPEB binds the small intracellular domain (ICD) of APLP1 and the related proteins APLP2 and APP. These proteins promote polyadenylation and translation by stimulating Aurora A catalyzed CPEB serine 174 phosphorylation. Surprisingly, CPEB, Maskin, CPSF, and several other factors involved in polyadenylation and translation and CPE-containing RNA are all detected on membranes by cell fractionation and immunoelectron microscopy. Moreover, most of the RNA that undergoes polyadenylation does so in membrane-containing fractions. These data demonstrate a link between cytoplasmic polyadenylation and membrane association and implicate APP family member proteins as anchors for localized mRNA polyadenylation and translation.
DOI of Published Version
Mol Cell Biol. 2005 Dec;25(24):10930-9. Link to article on publisher's site
Molecular and cellular biology
Cao Q, Huang Y, Kan M, Richter JD. (2005). Amyloid precursor proteins anchor CPEB to membranes and promote polyadenylation-induced translation. GSBS Student Publications. https://doi.org/10.1128/MCB.25.24.10930-10939.2005. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/182