GSBS Student Publications

Title

A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay

Student Author(s)

Christopher Merrikh

GSBS Program

Biochemistry & Molecular Pharmacology

Publication Date

2009-05-14

UMMS Affiliation

Graduate School of Biomedical Sciences, Biochemistry and Molecular Pharmacology Program

Document Type

Article

Disciplines

Genetics and Genomics | Molecular Biology

Abstract

Eukaryotes possess numerous quality control systems that monitor both the synthesis of RNA and the integrity of the finished products. We previously demonstrated that Saccharomyces cerevisiae possesses a quality control mechanism, nonfunctional rRNA decay (NRD), capable of detecting and eliminating translationally defective rRNAs. Here we show that NRD can be divided into two mechanistically distinct pathways: one that eliminates rRNAs with deleterious mutations in the decoding site (18S NRD) and one that eliminates rRNAs containing deleterious mutations in the peptidyl transferase center (25S NRD). 18S NRD is dependent on translation elongation and utilizes the same proteins as those participating in no-go mRNA decay (NGD). In cells that accumulate 18S NRD and NGD decay intermediates, both RNA types can be seen in P-bodies. We propose that 18S NRD and NGD are different observable outcomes of the same initiating event: a ribosome stalled inappropriately at a sense codon during translation elongation.

DOI of Published Version

10.1016/j.molcel.2009.04.017

Source

Mol Cell. 2009 May 14;34(4):440-50. doi: 10.1016/j.molcel.2009.04.017. Link to article on publisher's site

Journal/Book/Conference Title

Molecular cell

Related Resources

Link to Article in PubMed

PubMed ID

19481524

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