GSBS Student Publications

Title

Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

Student Author(s)

Ahmet Rasim Barutcu

GSBS Program

Interdisciplinary Graduate Program

Publication Date

2011-10-30

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine; Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Life Sciences | Medicine and Health Sciences

Abstract

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

DOI of Published Version

10.1038/nmeth.1764

Source

Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site

Journal/Book/Conference Title

Nature methods

Related Resources

Link to Article in PubMed

PubMed ID

22037702

Share

COinS