Yeast one-hybrid assays for gene-centered human gene regulatory network mapping
Interdisciplinary Graduate Program
Program in Gene Function and Expression; Program in Molecular Medicine; Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Life Sciences | Medicine and Health Sciences
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
DOI of Published Version
Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site
Reece-Hoyes JS, Barutcu AR, McCord RP, Jeong J, Jiang L, Macwilliams A, Yang X, Salehi-Ashtiani K, Hill DE, Blackshaw S, Zhu H, Dekker J, Walhout AJ. (2011). Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. GSBS Student Publications. https://doi.org/10.1038/nmeth.1764. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1763