Title
Twin-spot MARCM to reveal the developmental origin and identity of neurons
Academic Program
Neuroscience
UMMS Affiliation
Department of Neurobiology; Tzumin Lee Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Publication Date
2009-7
Document Type
Article
Disciplines
Neuroscience and Neurobiology
Abstract
A comprehensive understanding of the brain requires the analysis of individual neurons. We used twin-spot mosaic analysis with repressible cell markers (twin-spot MARCM) to trace cell lineages at high resolution by independently labeling paired sister clones. We determined patterns of neurogenesis and the influences of lineage on neuron-type specification. Notably, neural progenitors were able to yield intermediate precursors that create one, two or more neurons. Furthermore, neurons acquired stereotyped projections according to their temporal position in various brain sublineages. Twin-spot MARCM also permitted birth dating of mutant clones, enabling us to detect a single temporal fate that required chinmo in a sublineage of six Drosophila central complex neurons. In sum, twin-spot MARCM can reveal the developmental origins of neurons and the mechanisms that underlie cell fate.
DOI of Published Version
10.1038/nn.2345
Source
Nat Neurosci. 2009 Jul;12(7):947-53. Epub 2009 Jun 14. Link to article on publisher's site
Journal/Book/Conference Title
Nature neuroscience
Related Resources
PubMed ID
19525942
Repository Citation
Yu H, Chen C, Shi L, Huang Y, Lee T. (2009). Twin-spot MARCM to reveal the developmental origin and identity of neurons. GSBS Student Publications. https://doi.org/10.1038/nn.2345. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1720