GSBS Student Publications

Title

Preparation of late Drosophila embryonic fillets

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

Publication Date

2010-10-05

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Document Type

Article

Disciplines

Neuroscience and Neurobiology

Abstract

Beyond the age of ~15 h (at 25 degrees C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.

DOI of Published Version

10.1101/pdb.prot5498

Source

Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5498. doi: 10.1101/pdb.prot5498.

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

20889695

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