Student Authors
Preethi RamachandranAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2010-10-05Keywords
Animals; Cell Culture Techniques; Drosophila; Histological Techniques; OogoniaNeuroscience and Neurobiology
Metadata
Show full item recordAbstract
Beyond the age of ~15 h (at 25 degrees C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.Source
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5498. doi: 10.1101/pdb.prot5498.DOI
10.1101/pdb.prot5498Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33165PubMed ID
20889695Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot5498