Electron microscopy of Drosophila larval neuromuscular junctions
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.
DOI of Published Version
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.
Cold Spring Harbor protocols
Ramachandran P, Budnik V. (2010). Electron microscopy of Drosophila larval neuromuscular junctions. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1101/pdb.prot5474. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1703