Title
Electron microscopy of Drosophila larval neuromuscular junctions
Academic Program
Neuroscience
UMMS Affiliation
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Publication Date
2010-08-04
Document Type
Article
Disciplines
Neuroscience and Neurobiology
Abstract
Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.
DOI of Published Version
10.1101/pdb.prot5474
Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
20679383
Repository Citation
Ramachandran P, Budnik V. (2010). Electron microscopy of Drosophila larval neuromuscular junctions. GSBS Student Publications. https://doi.org/10.1101/pdb.prot5474. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1703