GSBS Student Publications

Title

Electron microscopy of Drosophila larval neuromuscular junctions

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

Publication Date

2010-08-04

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Document Type

Article

Disciplines

Neuroscience and Neurobiology

Abstract

Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.

DOI of Published Version

10.1101/pdb.prot5474

Source

Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

20679383

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