GSBS Student Publications
Title
Electron microscopy of Drosophila larval neuromuscular junctions
GSBS Program
Neuroscience
Publication Date
2010-08-04
UMMS Affiliation
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Document Type
Article
Disciplines
Neuroscience and Neurobiology
Abstract
Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.
DOI of Published Version
10.1101/pdb.prot5474
Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
20679383
Repository Citation
Ramachandran, Preethi and Budnik, Vivian, "Electron microscopy of Drosophila larval neuromuscular junctions" (2010). GSBS Student Publications. 1703.
https://escholarship.umassmed.edu/gsbs_sp/1703