Electron microscopy of Drosophila larval neuromuscular junctions
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Medical Subject Headings
Animals; Dissection; Drosophila; Larva; Microscopy, Electron; Muscles; Neuromuscular Junction
Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.
Rights and Permissions
Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.
DOI of Published Version
Cold Spring Harbor protocols
Ramachandran, Preethi and Budnik, Vivian, "Electron microscopy of Drosophila larval neuromuscular junctions" (2010). GSBS Student Publications. 1703.