Activity paradigm to induce Drosophila larval neuromuscular junction growth
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Neuroscience and Neurobiology
This article describes a method for spaced stimulation of Drosophilamotor neurons. The neurons can be stimulated for short periodsof time (~2 h) and then fixed (or imaged live). The method allowsone to examine the immediate effects of altering activity onsynaptic protein localization as well as neuromuscular junctionstructure and function.
DOI of Published Version
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5473. doi: 10.1101/pdb.prot5473.
Cold Spring Harbor protocols
Ramachandran, Preethi and Budnik, Vivian, "Activity paradigm to induce Drosophila larval neuromuscular junction growth" (2010). GSBS Student Publications. 1702.