Activity paradigm to induce Drosophila larval neuromuscular junction growth
Student Authors
Preethi RamachandranAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2010-08-04Keywords
Animals; Dissection; Drosophila melanogaster; Electrophysiology; Imaging, Three-Dimensional; Larva; Motor Activity; Neuromuscular Junction; Tissue FixationNeuroscience and Neurobiology
Metadata
Show full item recordAbstract
This article describes a method for spaced stimulation of Drosophilamotor neurons. The neurons can be stimulated for short periodsof time (~2 h) and then fixed (or imaged live). The method allowsone to examine the immediate effects of altering activity onsynaptic protein localization as well as neuromuscular junctionstructure and function.Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5473. doi: 10.1101/pdb.prot5473.DOI
10.1101/pdb.prot5473Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33163PubMed ID
20679382Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot5473