Fm1-43 labeling of Drosophila larval neuromuscular junctions
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscularjunction (NMJ) has gained immense popularity as a model systemfor the study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. Here, we describe a simple methodfor looking at vesicle recycling at the NMJ by labeling withFM1-43 dye.
DOI of Published Version
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471.
Cold Spring Harbor protocols
Ramachandran P, Budnik V. (2010). Fm1-43 labeling of Drosophila larval neuromuscular junctions. GSBS Student Publications. https://doi.org/10.1101/pdb.prot5471. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1700