GSBS Student Publications
Title
Fm1-43 labeling of Drosophila larval neuromuscular junctions
GSBS Program
Neuroscience
UMMS Affiliation
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Date
8-4-2010
Document Type
Article
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Larva; Neuromuscular Junction; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling
Disciplines
Neuroscience and Neurobiology
Abstract
Over the last two decades, the Drosophila larval neuromuscularjunction (NMJ) has gained immense popularity as a model systemfor the study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. Here, we describe a simple methodfor looking at vesicle recycling at the NMJ by labeling withFM1-43 dye.
Rights and Permissions
Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471.
DOI of Published Version
10.1101/pdb.prot5471
Related Resources
Journal Title
Cold Spring Harbor protocols
PubMed ID
20679380
Repository Citation
Ramachandran, Preethi and Budnik, Vivian, "Fm1-43 labeling of Drosophila larval neuromuscular junctions" (2010). GSBS Student Publications. 1700.
https://escholarship.umassmed.edu/gsbs_sp/1700