GSBS Student Publications

Student Author(s)

Caroline Connor

GSBS Program


Publication Date


UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology; Brudnick Neuropsychiatric Research Institute, Department of Psychiatry

Document Type



Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology


Antibodies differentiating between the mono-, di- and trimethylated forms of specific histone lysine residues are a critical tool in epigenome research, but show variable specificity, potentially limiting comparisons across studies and between samples. Using trimethyl histone H3 lysine 4 (H3K4me3)-a mark enriched at transcription start sites (TSS) of active genes-as an example, we describe how simple co-incubation with synthetic peptide of the K4me2 modification leads to increased specificity for K4me3 and a much sharper peak distribution proximal to TSS following chromatin immunoprecipitation and massively parallel sequencing (ChIP-Seq).

Rights and Permissions

This is an open access article licensed under a under a Creative Commons Attribution-NonCommercial 3.0 Unported License.

DOI of Published Version



Epigenetics. 2010 Jul 1;5(5):392-5. Epub 2010 Jul 1. Link to article on publisher's website

Journal/Book/Conference Title

Epigenetics : official journal of the DNA Methylation Society

Related Resources

Link to Article in PubMed

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.