GSBS Student Publications
Title
Regulated expression of the Escherichia coli dam gene
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Date
8-5-2003
Document Type
Article
Medical Subject Headings
ATP-Dependent Proteases; Arabinose; Culture Media; DNA Methylation; Escherichia coli; *Escherichia coli Proteins; *Gene Expression Regulation, Bacterial; Heat-Shock Proteins; Mutation; Promoter Regions (Genetics); *Protease La; Serine Endopeptidases; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Transcription, Genetic
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
Rights and Permissions
Citation: J Bacteriol. 2003 Aug;185(16):5012-4.
Related Resources
Journal Title
Journal of bacteriology
PubMed ID
12897023
Repository Citation
Calmann, Melissa A. and Marinus, Martin G., "Regulated expression of the Escherichia coli dam gene" (2003). GSBS Student Publications. 168.
https://escholarship.umassmed.edu/gsbs_sp/168