Title
Regulated expression of the Escherichia coli dam gene
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Publication Date
2003-08-05
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
DOI of Published Version
10.1128/JB.185.16.5012-5014.2003
Source
J Bacteriol. 2003 Aug;185(16):5012-4.
Journal/Book/Conference Title
Journal of bacteriology
Related Resources
PubMed ID
12897023
Repository Citation
Calmann MA, Marinus MG. (2003). Regulated expression of the Escherichia coli dam gene. GSBS Student Publications. https://doi.org/10.1128/JB.185.16.5012-5014.2003. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/168