GSBS Student Publications

Title

Regulated expression of the Escherichia coli dam gene

GSBS Program

Biochemistry & Molecular Pharmacology

Publication Date

2003-08-05

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

Source

J Bacteriol. 2003 Aug;185(16):5012-4.

Journal/Book/Conference Title

Journal of bacteriology

Related Resources

Link to article in PubMed

PubMed ID

12897023

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