GSBS Student Publications
Title
Regulated expression of the Escherichia coli dam gene
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2003-08-05
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
Source
J Bacteriol. 2003 Aug;185(16):5012-4.
Journal/Book/Conference Title
Journal of bacteriology
Related Resources
PubMed ID
12897023
Repository Citation
Calmann, Melissa A. and Marinus, Martin G., "Regulated expression of the Escherichia coli dam gene" (2003). GSBS Student Publications. 168.
https://escholarship.umassmed.edu/gsbs_sp/168