Interdisciplinary Graduate Program
Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences | Nucleic Acids, Nucleotides, and Nucleosides
Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.
DOI of Published Version
Conine CC, Batista PJ, Gu W, Claycomb JM, Chavez DA, Shirayama M, Mello CC (2010) The Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans. PNAS 107:3588-3593. Link to article on publisher's website
Proceedings of the National Academy of Sciences of the United States of America
Conine CC, Batista PJ, Gu W, Claycomb JM, Chaves DA, Shirayama M, Mello CC. (2010). Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans.. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1073/pnas.0911685107. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1638