Title

Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C.

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2009-11-13

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Exposure of cells to DNA-damaging agents results in a rapid increase in the formation of subnuclear complexes containing Rad51. To date, it has not been determined to what extent DNA damage-induced cytoplasmic to nuclear transport of Rad51 may contribute to this process. We have analyzed subcellular fractions of HeLa and HCT116 cells and found a significant increase in nuclear Rad51 levels following exposure to a modest dose of ionizing radiation (2 grays). We also observed a DNA damage-induced increase in nuclear Rad51 in the Brca2-defective cell line Capan-1. To address a possible Brca2-independent mechanism for Rad51 nuclear transport, we analyzed subcellular fractions for two other Rad51-interacting proteins, Rad51C and Xrcc3. Rad51C has a functional nuclear localization signal, and although we found that the subcellular distribution of Xrcc3 was not significantly affected by DNA damage, there was a damage-induced increase in nuclear Rad51C. Furthermore, RNA interference-mediated depletion of Rad51C in HeLa and Capan-1 cells resulted in lower steady-state levels of nuclear Rad51 as well as a diminished DNA damage-induced increase. Our results provide important insight into the cellular regulation of Rad51 nuclear entry and a role for Rad51C in this process.

DOI of Published Version

10.1074/jbc.M109.024646

Source

Gildemeister, O.G., Sage, J.M. and Knight, K.L. (2009) Cellular Redistribution of Rad51 in Response to DNA Damage: Novel Role for Rad51C. J. Biol. Chem. 284, 31945-31952.

Journal/Book/Conference Title

The Journal of biological chemistry

Related Resources

Link to article in PubMed

PubMed ID

19783859

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