GSBS Student Publications


Mechanism of synergistic induction of hepatic heme oxygenase by glutethimide and iron: studies in cultured chick embryo liver cells

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences



Document Type


Medical Subject Headings

Animals; Blotting, Northern; Blotting, Western; Chick Embryo; Drug Synergism; Enzyme Induction; Ferric Compounds; Glutethimide; Heme Oxygenase (Decyclizing); Heptanoates; Iron; Liver; Mixed Function Oxygenases; Nitrilotriacetic Acid; RNA, Messenger


Life Sciences | Medicine and Health Sciences


Heme oxygenase, the rate controlling enzyme for heme catabolism, is inducible by a variety of treatments, some of which induce by a heme-dependent mechanism and others by a heme-independent mechanism. This work shows that, in cultured chick embryo liver cells, synergistic induction of heme oxygenase by iron, added with the phenobarbital-like drug, glutethimide was heme-dependent. Addition of an inhibitor of heme biosynthesis abolished the synergistic induction of heme oxygenase providing evidence for the heme-dependent mechanism of induction. Glutethimide and iron appeared to induce at the transcriptional level since both heme oxygenase mRNA and protein levels correlate with changes in heme oxygenase activity.

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Citation: Biochem Biophys Res Commun. 1990 Apr 16;168(1):176-81.

Related Resources

Link to article in PubMed

Journal Title

Biochemical and biophysical research communications

PubMed ID