Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine; Department of Molecular Genetics and Microbiology; Program in Bioinformatics and Integrative Biology; Graduate School of Biomedical Sciences
Life Sciences | Medicine and Health Sciences
Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA (dsRNA) as a defense against viral infection. We identified endogenous siRNAs (endo-siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double-stranded RNA in vivo. Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2). We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line.
DOI of Published Version
Science. 2008 May 23;320(5879):1077-81. Epub 2008 Apr 10. Link to article on publisher's site
Science (New York, N.Y.)
Ghildiyal M, Seitz H, Horwich MD, Li C, Du T, Lee S, Xu J, Kittler EL, Zapp ML, Weng Z, Zamore PD. (2008). Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1126/science.1157396. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1606