GSBS Student Publications

Title

Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells

Publication Date

2008-04-12

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine; Department of Molecular Genetics and Microbiology; Program in Bioinformatics and Integrative Biology; Graduate School of Biomedical Sciences

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA (dsRNA) as a defense against viral infection. We identified endogenous siRNAs (endo-siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double-stranded RNA in vivo. Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2). We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line.

DOI of Published Version

10.1126/science.1157396

Source

Science. 2008 May 23;320(5879):1077-81. Epub 2008 Apr 10. Link to article on publisher's site

Journal/Book/Conference Title

Science (New York, N.Y.)

Related Resources

Link to Article in PubMed

PubMed ID

18403677

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