Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine; Department of Molecular Genetics and Microbiology; Program in Bioinformatics and Integrative Biology; Graduate School of Biomedical Sciences
Life Sciences | Medicine and Health Sciences
Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA (dsRNA) as a defense against viral infection. We identified endogenous siRNAs (endo-siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double-stranded RNA in vivo. Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2). We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line.
DOI of Published Version
Science. 2008 May 23;320(5879):1077-81. Epub 2008 Apr 10. Link to article on publisher's site
Science (New York, N.Y.)
Ghildiyal, Megha; Seitz, Herve; Horwich, Michael D.; Li, Chengjian; Du, Tingting; Lee, Soohyun; Xu, Jia; Kittler, Ellen L. W.; Zapp, Maria L.; Weng, Zhiping; and Zamore, Phillip D., "Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells" (2008). GSBS Student Publications. 1606.