GSBS Student Publications

UMMS Affiliation

Program in Molecular Medicine; Department of Physiology



Document Type


Medical Subject Headings

Animals; Carrier Proteins; Cell Differentiation; Cell Division; Centrosome; Chromosomes, Mammalian; Gene Deletion; Kidney; Kidney Diseases, Cystic; Mice; Mice, Knockout; Mitotic Spindle Apparatus; Wnt Proteins


Life Sciences | Medicine and Health Sciences


Primary cilia project from the surface of most vertebrate cells and are thought to be sensory organelles. Defects in primary cilia lead to cystic kidney disease, although the ciliary mechanisms that promote and maintain normal renal function remain incompletely understood. In this work, we generated a floxed allele of the ciliary assembly gene Ift20. Deleting this gene specifically in kidney collecting duct cells prevents cilia formation and promotes rapid postnatal cystic expansion of the kidney. Dividing collecting duct cells in early stages of cyst formation fail to properly orient their mitotic spindles along the tubule, whereas nondividing cells improperly position their centrosomes. At later stages, cells lacking cilia have increased canonical Wnt signaling and increased rates of proliferation. Thus, IFT20 functions to couple extracellular events to cell proliferation and differentiation.

Rights and Permissions

Citation: J Cell Biol. 2008 Nov 3;183(3):377-84. Link to article on publisher's site. Copyright remains with the authors.

DOI of Published Version


Related Resources

Link to article in PubMed

Journal Title

The Journal of cell biology

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.