Title
Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Cell Biology
Publication Date
2008-07-19
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT-PCR), has been reported in non-skeletal tissues such as breast, T cells and testis. To better define Runx2 activity in non-skeletal tissues, we examined transgenic (Tg) mice expressing LacZ gene under control of 3.0 kb (3 kb Tg) or 1.0 kb (1 kb Tg) of the Runx2 distal (P1) promoter, Runx2 LacZ knock-in (Runx2(+/LacZ)) and Runx2/P1 LacZ knock-in (Runx2/P1(+/LacZ)). In the Runx2 3 kb Tg mouse, beta-galactosidase (beta-gal) expression appeared in various non-skeletal tissues including testis, skin, adrenal gland and brain. beta-gal expression from both 3 kb and 1 kb Tg, reflecting activity of the Runx2 promoter, was readily detectable in seminiferous tubules of the testis and the epididymis. At the single cell level, beta-gal was detected in spermatids and mature sperms not in sertoli or Leydig cells. We also detected a positive signal from the Runx2(+/LacZ) and Runx2/P1(+/LacZ) mice. Indeed, Runx2 expression was observed in isolated mature sperms, which was confirmed by RT-PCR and Western blot analysis. Runx2, however, was not related to sex determination and sperm motility. Runx2 mediated beta-gal activity is also found robustly in the hippocampus and frontal lobe of the brain in Runx2(+/LacZ). Collectively, these results indicate that Runx2 is expressed in several non-skeletal tissues particularly sperms of testis and hippocampus of brain. It suggests that Runx2 may play an important role in male reproductive organ testis and brain.
DOI of Published Version
10.1002/jcp.21524
Source
J Cell Physiol. 2008 Nov;217(2):511-7. Link to article on publisher's site
Journal/Book/Conference Title
Journal of cellular physiology
Related Resources
PubMed ID
18636555
Repository Citation
Jeong J, Jin J, Kim H, Kang S, Liu JC, Lengner CJ, Otto F, Mundlos S, Stein JL, Van Wijnen AJ, Lian JB, Stein GS, Choi J. (2008). Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain. GSBS Student Publications. https://doi.org/10.1002/jcp.21524. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1543