Role for beta1 integrins in cortical osteocytes during acute musculoskeletal disuse
Graduate School of Biomedical Sciences; Bone and Signaling Laboratory
Life Sciences | Medicine and Health Sciences
The mammalian skeleton adjusts bone structure and strength in response to changes in mechanical loading, however the molecular and cellular mechanisms governing this process in vivo are unknown. Terminally differentiated osteoblasts, the osteocytes, are presumptive mechanosensory cells for bone, and cell culture studies demonstrate that beta1 integrins participate in mechanical signaling. To determine the role of beta1 integrins in osteoblasts in vivo, we used the Cre-lox system to delete beta1 integrin from cells committed to the osteoblast lineage. While pCol2.3 Cre-mediated recombination was widespread in bones from Colalpha1(I)-Cre+/beta1fl/fl conditional knockout mice (cKO), beta1 integrin protein was depleted from cortical osteocytes, but not from cancellous osteocytes or cells lining bone surfaces in adults. Bones from cKO mice that were normally loaded were similar in structure to WT littermates. However, hindlimb unloading of adult cKO mice for one week intended to cause bone loss (disuse osteopenia), resulted in unexpected, rapid changes in the geometry of cortical bone; hindlimb unloading increased the cross-sectional area, marrow area, and moments of inertia in cKO, but not WT mice. Furthermore, these hindlimb unloading-induced geometric changes in cortical bone of cKO mice resulted in increased whole bone bending stiffness and strength of the femur. Together, these results confirmed the concept that osteocytes are mechanosensory cells and showed beta1 integrins in cortical osteocytes limited changes in cortical geometry in response to disuse, thus providing the first in vivo evidence that beta1 integrins on osteocytes mediate specific aspects of mechanotransduction.
DOI of Published Version
Matrix Biol. 2008 Sep;27(7):609-18. Epub 2008 Jun 20. Link to article on publisher's site
Matrix biology : journal of the International Society for Matrix Biology
Phillips, Jonathan Adam; Almeida, Eduardo A. C.; Hill, Esther L.; Aguirre, J. Ignacio; Rivera, Mercedes F.; Nachbandi, Inaam; Wronski, Thomas J.; van der Meulen, Marjolein C. H.; and Globus, Ruth K., "Role for beta1 integrins in cortical osteocytes during acute musculoskeletal disuse" (2008). GSBS Student Publications. 1537.