Regulation of cytokine production by virus-specific CD8 T cells in the lungs
UMass Chan Affiliations
Program in Immunology/VirologyDepartment of Microbiology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2008-06-06Keywords
Animals; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Female; Immunologic Memory; Inflammation; Interferon-gamma; Lung; Lymph Nodes; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Spleen; Tumor Necrosis Factor-alphaLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Inflammation and the elimination of infected host cells during an immune response often cause local tissue injury and immunopathology, which can disrupt the normal functions of tissues such as the lung. Here, we show that both virus-induced inflammation and the host tissue environment combine to influence the capacity of virus-specific CD4 and CD8 T cells to produce cytokines in various tissues. Decreased production of cytokines, such as IFN-gamma and TNF-alpha, by antigen-specific T cells is more pronounced in peripheral tissues, such as the lung and kidney, than in secondary lymphoid organs, such as the spleen or lymph nodes. We also demonstrate that tissues regulate cytokine production by memory T cells independently of virus infection, as memory T cells that traffic into the lungs of naive animals exhibit a reduced ability to produce cytokines following direct ex vivo peptide stimulation. Furthermore, we show that cytokine production by antigen-specific memory CD4 and CD8 T cells isolated from the lung parenchyma can be rescued by stimulation with exogenous peptide-pulsed antigen-presenting cells. Our results suggest that the regulation of T-cell cytokine production by peripheral tissues may serve as an important mechanism to prevent immunopathology and preserve normal tissue function.Source
J Virol. 2008 Aug;82(16):7799-811. Epub 2008 Jun 4. Link to article on publisher's siteDOI
10.1128/JVI.00840-08Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32961PubMed ID
18524828Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1128/JVI.00840-08