Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Life Sciences | Medicine and Health Sciences
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
DOI of Published Version
Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11. Link to article on publisher's site
Juette MF, Gould TJ, Lessard MD, Mlodzianoski MJ, Nagpure BS, Bennett BT, Hess ST, Bewersdorf J. (2008). Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples. GSBS Student Publications. https://doi.org/10.1038/nmeth.1211. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1500