GSBS Student Publications

Title

In situ nuclear organization of regulatory machinery

Publication Date

2008-05-09

UMMS Affiliation

Department of Cell Biology

Document Type

Article

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

DOI of Published Version

10.1007/978-1-59745-104-8_17

Source

Methods Mol Biol. 2008;455:239-59. Link to article on publisher's site

Journal/Book/Conference Title

Methods in molecular biology (Clifton, N.J.)

Related Resources

Link to Article in PubMed

PubMed ID

18463823

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