Title
Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing
UMMS Affiliation
Graduate School of Biomedical Sciences; Immune Disease Institute
Publication Date
2008-04-29
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Eri1 is a 3'-to-5' exoribonuclease conserved from fission yeast to humans. Here we show that Eri1 associates with ribosomes and ribosomal RNA (rRNA). Ribosomes from Eri1-deficient mice contain 5.8S rRNA that is aberrantly extended at its 3' end, and Eri1, but not a catalytically inactive mutant, converts this abnormal 5.8S rRNA to the wild-type form in vitro and in cells. In human and murine cells, Eri1 localizes to the cytoplasm and nucleus, with enrichment in the nucleolus, the site of preribosome biogenesis. RNA binding residues in the Eri1 SAP and linker domains promote stable association with rRNA and thereby facilitate 5.8S rRNA 3' end processing. Taken together, our findings indicate that Eri1 catalyzes the final trimming step in 5.8S rRNA processing, functionally and spatially connecting this regulator of RNAi with the basal translation machinery.
DOI of Published Version
10.1038/nsmb.1417
Source
Nat Struct Mol Biol. 2008 May;15(5):523-30. Epub 2008 Apr 27. Link to article on publisher's site
Journal/Book/Conference Title
Nature structural and molecular biology
Related Resources
PubMed ID
18438418
Repository Citation
Ansel KM, Pastor WA, Rath N, Lapan AD, Glasmacher E, Wolf C, Smith LC, Papadopoulou N, Lamperti ED, Tahiliani M, Ellwart JW, Shi Y, Kremmer E, Rao A, Heissmeyer V. (2008). Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing. GSBS Student Publications. https://doi.org/10.1038/nsmb.1417. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1491