Utilization of Fc receptors as a mucosal vaccine strategy against an intracellular bacterium, Francisella tularensis
Graduate School of Biomedical Sciences; Program in Immunology and Virology
Medical Subject Headings
Administration, Intranasal; Animals; Antibodies, Bacterial; Bacterial Vaccines; Cytokines; Francisella tularensis; Immunity, Mucosal; Immunization, Secondary; Immunoglobulin A; Immunologic Memory; Inflammation; Interferon-gamma; Liver; Lung; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mucous Membrane; Receptors, Fc; Spleen; Tularemia; Vaccination
Life Sciences | Medicine and Health Sciences
Numerous studies have demonstrated that targeting Ag to Fc receptors (FcR) on APCs can enhance humoral and cellular immunity. However, studies are lacking that examine both the use of FcR-targeting in generating immune protection against infectious agents and the use of FcRs in the induction of mucosal immunity. Francisella tularensis is a category A intracellular mucosal pathogen. Thus, intense efforts are underway to develop a vaccine against this organism. We hypothesized that protection against mucosal infection with F. tularensis would be significantly enhanced by targeting inactivated F. tularensis live vaccine strain (iFt) to FcRs at mucosal sites, via intranasal immunization with mAb-iFt complexes. These studies demonstrate for the first time that: 1) FcR-targeted immunogen enhances immunogen-specific IgA production and protection against subsequent infection in an IgA-dependent manner, 2) FcgammaR and neonatal FcR are crucial to this protection, and 3) inactivated F. tularensis, when targeted to FcRs, enhances protection against the highly virulent SchuS4 strain of F. tularensis, a category A biothreat agent. In summary, these studies show for the first time the use of FcRs as a highly effective vaccination strategy against a highly virulent mucosal intracellular pathogen.
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Citation: J Immunol. 2008 Apr 15;180(8):5548-57.
Journal of immunology (Baltimore, Md. : 1950)
Rawool, Deepak B.; Bitsaktsis, Constantine; Li, Ying; Gosselin, Diane R.; Lin, Yili; Kurkure, Nitin V.; Metzger, Dennis W.; and Gosselin, Edmund J., "Utilization of Fc receptors as a mucosal vaccine strategy against an intracellular bacterium, Francisella tularensis" (2008). GSBS Student Publications. 1475.