GSBS Student Publications

Title

Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay

Publication Date

2008-02-26

UMMS Affiliation

Graduate School of Biomedical Sciences; Vaccine Branch

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge.

DOI of Published Version

10.1016/j.jim.2008.01.008

Source

J Immunol Methods. 2008 Apr 20;333(1-2):71-8. Epub 2008 Feb 8. Link to article on publisher's site

Journal/Book/Conference Title

Journal of immunological methods

Related Resources

Link to Article in PubMed

PubMed ID

18294650

Share

COinS