Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay
Graduate School of Biomedical Sciences; Vaccine Branch
Life Sciences | Medicine and Health Sciences
Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge.
DOI of Published Version
J Immunol Methods. 2008 Apr 20;333(1-2):71-8. Epub 2008 Feb 8. Link to article on publisher's site
Journal of immunological methods
Dzutsev AK, Belyakov IM, Isakov DV, Gagnon SJ, Margulies DH, Berzofsky JA. (2008). Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay. GSBS Student Publications. https://doi.org/10.1016/j.jim.2008.01.008. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1458