GSBS Student Publications
Title
Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay
Publication Date
2008-02-26
UMMS Affiliation
Graduate School of Biomedical Sciences; Vaccine Branch
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge.
DOI of Published Version
10.1016/j.jim.2008.01.008
Source
J Immunol Methods. 2008 Apr 20;333(1-2):71-8. Epub 2008 Feb 8. Link to article on publisher's site
Journal/Book/Conference Title
Journal of immunological methods
Related Resources
PubMed ID
18294650
Repository Citation
Dzutsev, Amiran K.; Belyakov, Igor M.; Isakov, Dmitry V.; Gagnon, Susan J.; Margulies, David H.; and Berzofsky, Jay A., "Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay" (2008). GSBS Student Publications. 1458.
https://escholarship.umassmed.edu/gsbs_sp/1458