Title
IL-13 is required for eosinophil entry into the lung during respiratory syncytial virus vaccine-enhanced disease
Academic Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Interdisciplinary Graduate Program in Immunology
Publication Date
2008-02-06
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in children. Children previously vaccinated with a formalin-inactivated RSV vaccine experienced enhanced morbidity and mortality upon natural RSV infection. Histological analysis revealed the presence of eosinophils in the pulmonary infiltrate of the vaccinated children. Eosinophils are characteristic of Th2 responses, and Th2 cells are known to be necessary to induce pulmonary eosinophilia in RSV-infected BALB/c mice previously immunized with a recombinant vaccinia virus (vv) expressing the RSV G protein (vvG). Using IL-13-deficient mice, we find that IL-13 is necessary for eosinophils to reach the lung parenchyma and airways of vvG-immunized mice undergoing RSV challenge infection. IL-13 acts specifically on eosinophils as the magnitude of pulmonary inflammation, RSV G protein-specific CD4 T cell responses, and virus clearance were not altered in IL-13-deficient mice. After RSV challenge, eosinophils were readily detectable in the blood and bone marrow of vvG-immunized IL-13-deficient mice, suggesting that IL-13 is required for eosinophils to transit from the blood into the lung. Pulmonary levels of CCL11 and CCL22 protein were significantly reduced in IL-13-deficient mice indicating that IL-13 mediates the recruitment of eosinophils into the lungs by inducing the production of chemokines important in Th2 cell and eosinophil chemotaxis.
DOI of Published Version
10.4049/jimmunol.180.4.2376
Source
J Immunol. 2008 Feb 15;180(4):2376-84.
Journal/Book/Conference Title
Journal of immunology (Baltimore, Md. : 1950)
Related Resources
PubMed ID
18250447
Repository Citation
Castilow EM, Meyerholz DK, Varga SM. (2008). IL-13 is required for eosinophil entry into the lung during respiratory syncytial virus vaccine-enhanced disease. GSBS Student Publications. https://doi.org/10.4049/jimmunol.180.4.2376. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1444