Alu elements mediate MYB gene tandem duplication in human T-ALL
Department of Pediatric Oncology; Department of Molecular Genetics and Microbiology
Life Sciences | Medicine and Health Sciences
Recent studies have demonstrated that the MYB oncogene is frequently duplicated in human T cell acute lymphoblastic leukemia (T-ALL). We find that the human MYB locus is flanked by 257-bp Alu repeats and that the duplication is mediated somatically by homologous recombination between the flanking Alu elements on sister chromatids. Nested long-range PCR analysis indicated a low frequency of homologous recombination leading to MYB tandem duplication in the peripheral blood mononuclear cells of approximately 50% of healthy individuals, none of whom had a MYB duplication in the germline. We conclude that Alu-mediated MYB tandem duplication occurs at low frequency during normal thymocyte development and is clonally selected during the molecular pathogenesis of human T-ALL.
DOI of Published Version
J Exp Med. 2007 Dec 24;204(13):3059-66. Epub 2007 Dec 10. Link to article on publisher's site
The Journal of experimental medicine
O'Neil JE, Tchinda J, Gutierrez A, Moreau LA, Maser RS, Wong K, Li W, McKenna K, Liu XS, Feng B, Neuberg DS, Silverman LB, DeAngelo DJ, Kutok JL, Rothstein R, DePinho RA, Chin L, Lee C, Look AT. (2007). Alu elements mediate MYB gene tandem duplication in human T-ALL. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1084/jem.20071637. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1420