Mus81, Rhp51(Rad51) and Rqh1 Form an Epistatic Pathway Required for the S-phase DNA Damage Checkpoint
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2008-11-28
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Show full item recordAbstract
Monitoring Editor: Orna Cohen-Fix The S-phase DNA damage checkpoint slows the rate of DNA synthesis in response to damage during replication. In the fission yeast Schizosaccharomyces pombe, Cds1, the S-phase specific checkpoint effector kinase, is required for checkpoint signaling and replication slowing; upon treatment with the alkylating agent MMS, cds1Delta mutants display a complete checkpoint defect. We have identified proteins downstream of Cds1 required for checkpoint-dependant slowing, including the structure-specific endonuclease Mus81 and the helicase Rqh1, which are implicated in replication fork stability and the negative regulation of recombination. Removing Rhp51, the Rad51 recombinase homolog, suppresses the slowing defect of rqh1Delta mutants, but not that of mus81Delta mutant, defining an epistatic pathway in which mus81 is epistatic to rhp51 and rhp51 is epistatic to rqh1. We propose that restraining recombination is required for the slowing of replication in response to DNA damage.Source
Mol Biol Cell. 2008 Nov 26. Link to article on publisher's siteDOI
10.1091/mbc.E08-08-0798Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32828PubMed ID
19037101Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1091/mbc.E08-08-0798