GSBS Student Publications


Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology; Department of Molecular Genetics and Microbiology



Document Type


Medical Subject Headings

Animals; Cell Line; Cercopithecus aethiops; Cricetinae; Cryopreservation; *Culture Techniques; Lymphocytic choriomeningitis virus; Occupational Health; Plaque Assay; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Vero Cells; Virology; Virus Cultivation


Life Sciences | Medicine and Health Sciences


Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus-induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.

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Citation: Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. Link to article on publisher's site

DOI of Published Version


Related Resources

Link to Article in PubMed

Journal Title

Current protocols in microbiology

PubMed ID