Calcium gradients underlying polarization and chemotaxis of eosinophils
Document Type
Journal ArticlePublication Date
1991-11-01Keywords
Calcium; *Chemotaxis, Leukocyte; Eosinophils; Fura-2; Humans; Ionomycin; Microscopy, Fluorescence; Microscopy, Phase-ContrastLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The concentration of intracellular free calcium ([Ca2+]i) in polarized eosinophils was imaged during chemotaxis by monitoring fluorescence of the calcium-sensitive dye Fura-2 with a modified digital imaging microscope. Chemotactic stimuli caused [Ca2+]i to increase in a nonuniform manner that was related to cell activity. In cells moving persistently in one direction, [Ca2+]i was highest at the rear and lowest at the front of the cell. Before cells turned, [Ca2+]i transiently increased. The region of the cell that became the new leading edge had the lowest [Ca2+]i. These changes in [Ca2+]i provide a basis for understanding the organization and local activity of cytoskeletal proteins thought to underlie the directed migration of many cells.Source
Science. 1991 Nov 1;254(5032):703-6.
DOI
10.1126/science.1948048Permanent Link to this Item
http://hdl.handle.net/20.500.14038/32806PubMed ID
1948048Related Resources
ae974a485f413a2113503eed53cd6c53
10.1126/science.1948048