GSBS Student Publications
Title
Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Psychiatry
Date
4-3-2008
Document Type
Article
Medical Subject Headings
Animals; Blotting, Northern; Blotting, Western; Brain; Cell Line; *Gene Expression Regulation; Immunoprecipitation; Mice; Neurons; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Promoter Regions, Genetic; Rats; Receptors, Nicotinic; Transcription Factors; Transcription, Genetic
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Nicotinic acetylcholine receptors are involved in a plethora of fundamental biological processes ranging from muscle contraction to formation of memories. The receptors are pentameric proteins whose subunits are encoded by distinct genes. Subunit composition of a mature nicotinic receptor is governed in part by the transcriptional regulation of each subunit gene. Here, using chromatin immunoprecipitation assays, we report the interaction of the transcription factors Sp1, Sp3, c-Jun and Sox10 with the beta4 subunit gene promoter in neuronal-like cell lines and rodent brain tissue. Our results corroborate previous in-vitro data demonstrating that these transcription factors interact with the beta4 promoter. Taken together, these data suggest that Sp1, Sp3, c-Jun and Sox10 regulate expression of the beta4 subunit gene in the mammalian brain.
Rights and Permissions
Citation: Neuroreport. 2008 Apr 16;19(6):687-90. Link to article on publisher's site
DOI of Published Version
10.1097/WNR.0b013e3282fbcef7
Related Resources
Journal Title
Neuroreport
PubMed ID
18382288
Repository Citation
Scofield, Michael D.; Bruschweiler-Li, Lei; Mou, Zhongming; and Gardner, Paul D., "Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter" (2008). GSBS Student Publications. 1359.
https://escholarship.umassmed.edu/gsbs_sp/1359