Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter
Graduate School of Biomedical Sciences; Department of Psychiatry
Life Sciences | Medicine and Health Sciences
Nicotinic acetylcholine receptors are involved in a plethora of fundamental biological processes ranging from muscle contraction to formation of memories. The receptors are pentameric proteins whose subunits are encoded by distinct genes. Subunit composition of a mature nicotinic receptor is governed in part by the transcriptional regulation of each subunit gene. Here, using chromatin immunoprecipitation assays, we report the interaction of the transcription factors Sp1, Sp3, c-Jun and Sox10 with the beta4 subunit gene promoter in neuronal-like cell lines and rodent brain tissue. Our results corroborate previous in-vitro data demonstrating that these transcription factors interact with the beta4 promoter. Taken together, these data suggest that Sp1, Sp3, c-Jun and Sox10 regulate expression of the beta4 subunit gene in the mammalian brain.
DOI of Published Version
Neuroreport. 2008 Apr 16;19(6):687-90. Link to article on publisher's site
Scofield MD, Bruschweiler-Li L, Mou Z, Gardner PD. (2008). Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1097/WNR.0b013e3282fbcef7. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1359