GSBS Student Publications


The ratio of mouse insulin I:insulin II does not reflect that of the corresponding preproinsulin mRNAs

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Neurology



Document Type


Medical Subject Headings

Amino Acid Sequence; Animals; Aspergillus Nuclease S1; Base Sequence; Chromatography, High Pressure Liquid; Culture Techniques; Eating; Fasting; Glucose; Insulin; Islets of Langerhans; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Molecular Sequence Data; Proinsulin; Protein Precursors; RNA, Messenger


Life Sciences | Medicine and Health Sciences


Rats and mice both express two, non-allelic, insulin genes. In the rat the ratio of the two preproinsulin mRNAs closely matches that of the mature insulin peptides. The experiments reported here demonstrate that this is not the case in the mouse. The relative amounts of the two murine proinsulin RNAs were measured by an S1 nuclease assay. The ratio of preproinsulin I mRNA to preproinsulin II mRNA was 4:1 in RNA extracted from the pancreas of mice fed ad libitum or fasted for 72 h. A similar value was found in mouse islets of Langerhans after maintenance in tissue culture for 48 h at either 2.8 or 16.7 mM glucose. The ratio of insulin I:insulin II peptides, assessed by separating the two insulins using reversed phase high-performance liquid chromatography, was approximately 1:3 in both pancreas and islets. Thus in the mouse, unlike the rat, the ratio of the two insulin peptides does not reflect that of the two preproinsulin mRNAs.

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Citation: Mol Cell Endocrinol. 1992 Aug;86(3):177-86.

Related Resources

Link to Article in PubMed

Journal Title

Molecular and cellular endocrinology

PubMed ID