Target accessibility dictates the potency of human RISC
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Base Sequence; Binding Sites; Catalysis; Cyclin-Dependent Kinase 9; HIV Long Terminal Repeat; Hela Cells; Humans; Kinetics; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Viral; RNA-Induced Silencing Complex
Life Sciences | Medicine and Health Sciences
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
Rights and Permissions
Citation: Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's site
DOI of Published Version
Nature structural and molecular biology
Brown, Kirk M.; Chu, Chia-ying; and Rana, Tariq M., "Target accessibility dictates the potency of human RISC" (2005). GSBS Student Publications. 133.