Title
Target accessibility dictates the potency of human RISC
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Publication Date
2005-04-27
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
DOI of Published Version
10.1038/nsmb931
Source
Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's site
Journal/Book/Conference Title
Nature structural and molecular biology
Related Resources
PubMed ID
15852021
Repository Citation
Brown KM, Chu C, Rana TM. (2005). Target accessibility dictates the potency of human RISC. GSBS Student Publications. https://doi.org/10.1038/nsmb931. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/133