Target accessibility dictates the potency of human RISC
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Life Sciences | Medicine and Health Sciences
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
DOI of Published Version
Nat Struct Mol Biol. 2005 May;12(5):469-70. Epub 2005 Apr 24. Link to article on publisher's site
Nature structural and molecular biology
Brown KM, Chu C, Rana TM. (2005). Target accessibility dictates the potency of human RISC. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1038/nsmb931. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/133