Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state
Graduate School of Biomedical Sciences; Howard Hughes Medical Institute; Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences
TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)s). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. At high cell density or following nutrient deprivation, yeast cells cease division, enter a G0-like state, and terminate transcription of most genes. In this stationary phase, we find that the levels of yTAF(II)145, several other yTAF(II)s, and TBP are drastically reduced. Collectively, our results indicate that yTAF(II)145 and other TFIID components have a specialized role in transcriptional regulation of cell cycle progression and growth control.
DOI of Published Version
Cell. 1997 Aug 22;90(4):607-14.
Walker SS, Shen W, Reese JC, Apone LM, Green MR. (1997). Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1016/S0092-8674(00)80522-X. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1325