GSBS Student Publications

Title

The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus

Publication Date

1992-10-30

UMMS Affiliation

Department of Cell Biology

Document Type

Article

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

X chromosome inactivation in mammalian females results in the cis-limited transcriptional inactivity of most of the genes on one X chromosome. The XIST gene is unique among X-linked genes in being expressed exclusively from the inactive X chromosome. Human XIST cDNAs containing at least eight exons and totaling 17 kb have been isolated and sequenced within the region on the X chromosome known to contain the X inactivation center. The XIST gene includes several tandem repeats, the most 5' of which are evolutionarily conserved. The gene does not contain any significant conserved ORFs and thus does not appear to encode a protein, suggesting that XIST may function as a structural RNA within the nucleus. Consistent with this, fluorescence in situ hybridization experiments demonstrate localization of XIST RNA within the nucleus to a position indistinguishable from the X inactivation-associated Barr body.

Source

Cell. 1992 Oct 30;71(3):527-42.

Journal/Book/Conference Title

Cell

Related Resources

Link to article in PubMed

PubMed ID

1423611

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