Title
HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Pathology; Program in Molecular Medicine
Publication Date
2001-12-26
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The p300/CBP family of transcriptional coactivators possesses multiple functional domains, including a histone acetyltransferase (HAT) and several activation domains. A number of models have been proposed to account for their roles in transcriptional activation, including interactions with basal transcription machinery and chromatin remodeling. However, individual contributions of these domains to transcriptional activation and their significance in living organisms remain unclear. We addressed the importance of the HAT activity of CBP-1, the worm ortholog of p300/CBP, in Caenorhabditis elegans with three different and complementary approaches. These include allele-specific RNA-mediated interference (RNAi), genetic rescue and the use of a specific chemical inhibitor of the p300/CBP HAT. Our findings demonstrate that HAT activity is of primary importance for CBP-1 to regulate transcription and to promote differentiation during C. elegans embryogenesis.
DOI of Published Version
10.1093/embo-reports/kvf006
Source
EMBO Rep. 2002 Jan;3(1):50-5. Epub 2001 Dec 19. Link to article on publisher's site
Journal/Book/Conference Title
EMBO reports
Related Resources
PubMed ID
11751575
Repository Citation
Victor M, Bei Y, Gay F, Calvo D, Mello CC, Shi Y. (2001). HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans. GSBS Student Publications. https://doi.org/10.1093/embo-reports/kvf006. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1313