Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Program in Gene Function and Expression
Life Sciences | Medicine and Health Sciences
The dynamic structure of individual nucleosomes was examined by stretching nucleosomal arrays with a feedback-enhanced optical trap. Forced disassembly of each nucleosome occurred in three stages. Analysis of the data using a simple worm-like chain model yields 76 bp of DNA released from the histone core at low stretching force. Subsequently, 80 bp are released at higher forces in two stages: full extension of DNA with histones bound, followed by detachment of histones. When arrays were relaxed before the dissociated state was reached, nucleosomes were able to reassemble and to repeat the disassembly process. The kinetic parameters for nucleosome disassembly also have been determined.
DOI of Published Version
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1960-5. Link to article on publisher's site
Proceedings of the National Academy of Sciences of the United States of America
Brower-Toland, Brent D.; Smith, Corey Lewis; Yeh, Richard C.; Lis, John T.; Peterson, Craig L.; and Wang, Michelle D., "Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA" (2002). GSBS Student Publications. 131.