Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2
Graduate School of Biomedical Sciences; Department of Cell Biology
Life Sciences | Medicine and Health Sciences
The human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase. The cell-cycle element (CCE) required for H4 histone activation is a sequence of 11 base pairs that binds a protein factor in electrophoretic mobility shift assays that has been designated histone nuclear factor M (HiNF-M). Here we report the purification of HiNF-M, and show it to be a protein of relative molecular mass (M(r)) 48K that is identical to interferon (IFN) regulatory factor 2 (IRF-2), a negative transcriptional regulator of the IFN response. Recombinant IRF-2 (as well as the related protein IRF-1 (ref. 5)) binds the CCE specifically and activates transcription of this H4 histone gene. IRF-2 has been shown to have oncogenic potential, and our results demonstrate a link between IRF-2 and a gene that is functionally coupled to DNA replication and cell-cycle progression at the G1/S phase transition.
DOI of Published Version
Nature. 1995 Sep 28;377(6547):362-5. Link to article on publisher's site
Vaughan, Patricia S.; Aziz, Farah; Van Wijnen, Andre J.; Wu, Shujian; Harada, Hisashi; Taniguchi, Tadatsugu; Soprano, Kenneth J.; Stein, Janet L.; and Stein, Gary S., "Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2" (1995). GSBS Student Publications. 1306.