Title
Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Publication Date
1995-09-28
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase. The cell-cycle element (CCE) required for H4 histone activation is a sequence of 11 base pairs that binds a protein factor in electrophoretic mobility shift assays that has been designated histone nuclear factor M (HiNF-M). Here we report the purification of HiNF-M, and show it to be a protein of relative molecular mass (M(r)) 48K that is identical to interferon (IFN) regulatory factor 2 (IRF-2), a negative transcriptional regulator of the IFN response. Recombinant IRF-2 (as well as the related protein IRF-1 (ref. 5)) binds the CCE specifically and activates transcription of this H4 histone gene. IRF-2 has been shown to have oncogenic potential, and our results demonstrate a link between IRF-2 and a gene that is functionally coupled to DNA replication and cell-cycle progression at the G1/S phase transition.
DOI of Published Version
10.1038/377362a0
Source
Nature. 1995 Sep 28;377(6547):362-5. Link to article on publisher's site
Journal/Book/Conference Title
Nature
Related Resources
PubMed ID
7566094
Repository Citation
Vaughan PS, Aziz F, Van Wijnen AJ, Wu S, Harada H, Taniguchi T, Soprano KJ, Stein JL, Stein GS. (1995). Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2. GSBS Student Publications. https://doi.org/10.1038/377362a0. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1306