In vivo occupancy of the vitamin D responsive element in the osteocalcin gene supports vitamin D-dependent transcriptional upregulation in intact cells
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Animals; Base Sequence; Binding Sites; Calcitriol; DNA-Binding Proteins; Gene Expression Regulation; Molecular Sequence Data; Oligodeoxyribonucleotides; Osteocalcin; Osteosarcoma; Promoter Regions (Genetics); RNA, Messenger; Rats; Receptors, Calcitriol; Transcription, Genetic; Tumor Cells, Cultured
Life Sciences | Medicine and Health Sciences
The steroid hormone vitamin D is a principal mediator of skeletal homeostasis. 1,25-Dihydroxyvitamin D3 treatment of ROS 17/2.8 osteoblast-like cells results in a ligand-dependent increase in transcription of the bone-specific osteocalcin gene. This transcriptional upregulation requires the positive cis-acting vitamin D responsive element (VDRE). We have used the ligation-mediated polymerase chain reaction to demonstrate that protein occupancy of the VDRE within the intact cell correlates with increased synthesis of osteocalcin transcripts. These protein-DNA contacts were not present in the absence of vitamin D or in osteosarcoma cells (ROS 24.1) lacking the vitamin D receptor. Our results establish in intact cells the requirement for both ligand- and receptor-dependent occupancy of the VDRE for vitamin D responsive enhancement of osteocalcin gene transcription.
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Citation: Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12902-6.
Proceedings of the National Academy of Sciences of the United States of America
Breen, Ellen C.; Van Wijnen, Andre J.; Lian, Jane B.; Stein, Gary S.; and Stein, Janet L., "In vivo occupancy of the vitamin D responsive element in the osteocalcin gene supports vitamin D-dependent transcriptional upregulation in intact cells" (1994). GSBS Student Publications. 129.