GSBS Student Publications
Title
Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor
GSBS Program
Not applicable
Publication Date
1988-08-23
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Vertebrate histone gene promoters in many cases contain an upstream element, 5'dCCAAT, that has been implicated in modulating the efficiency of transcription of a broad spectrum of genes. We have previously isolated a nuclear factor (HiNF-B) that binds specifically to the CCAAT element of a cell cycle regulated human H1 histone gene. This factor shows similarities with other CCAAT box binding proteins in that it recognizes the same sequence but shows a distinct chromatographic behavior. In the present study, we have employed the gel retardation assay to demonstrate that HiNF-B is a cell cycle independent DNA binding protein that is conserved in both human and mouse cells. Using a series of reconstitution experiments with partially purified HiNF-B fractions, we show that this factor requires association of at least two components for site-specific binding. The composite structure of HiNF-B suggests that binding of at least some CCAAT elements in vertebrates may require cooperative interaction of CCAAT box binding proteins with other factors.
DOI of Published Version
10.1021/bi00417a051
Source
Biochemistry. 1988 Aug 23;27(17):6534-41.
Journal/Book/Conference Title
Biochemistry
Related Resources
PubMed ID
3219352
Repository Citation
Van Wijnen AJ, Massung RF, Stein JL, Stein GS. (1988). Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor. GSBS Student Publications. https://doi.org/10.1021/bi00417a051. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1289