Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor
Graduate School of Biomedical Sciences; Department of Cell Biology
Life Sciences | Medicine and Health Sciences
Vertebrate histone gene promoters in many cases contain an upstream element, 5'dCCAAT, that has been implicated in modulating the efficiency of transcription of a broad spectrum of genes. We have previously isolated a nuclear factor (HiNF-B) that binds specifically to the CCAAT element of a cell cycle regulated human H1 histone gene. This factor shows similarities with other CCAAT box binding proteins in that it recognizes the same sequence but shows a distinct chromatographic behavior. In the present study, we have employed the gel retardation assay to demonstrate that HiNF-B is a cell cycle independent DNA binding protein that is conserved in both human and mouse cells. Using a series of reconstitution experiments with partially purified HiNF-B fractions, we show that this factor requires association of at least two components for site-specific binding. The composite structure of HiNF-B suggests that binding of at least some CCAAT elements in vertebrates may require cooperative interaction of CCAAT box binding proteins with other factors.
DOI of Published Version
Biochemistry. 1988 Aug 23;27(17):6534-41.
Van Wijnen AJ, Massung RF, Stein JL, Stein GS. (1988). Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor. GSBS Student Publications. https://doi.org/10.1021/bi00417a051. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1289