Protein/DNA interactions involving ATF/AP1-, CCAAT-, and HiNF-D-related factors in the human H3-ST519 histone promoter: cross-competition with transcription regulatory sites in cell cycle controlled H4 and H1 histone genes
Graduate School of Biomedical Sciences; Department of Cell Biology
Medical Subject Headings
Activating Transcription Factors; Base Sequence; Binding Sites; Binding, Competitive; Blood Proteins; CCAAT-Enhancer-Binding Proteins; Cell Cycle; DNA; DNA Fingerprinting; DNA Probes; DNA-Binding Proteins; Deoxyribonuclease I; *Gene Expression Regulation; Histones; Humans; Methylation; Molecular Sequence Data; *Promoter Regions (Genetics); Sulfuric Acid Esters; Transcription Factors; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Protein/DNA interactions of the H3-ST519 histone gene promoter were analyzed in vitro. Using several assays for sequence specificity, we established binding sites for ATF/AP1-, CCAAT-, and HiNF-D related DNA binding proteins. These binding sites correlate with two genomic protein/DNA interaction domains previously established for this gene. We show that each of these protein/DNA interactions has a counterpart in other histone genes: H3-ST519 and H4-F0108 histone genes interact with ATF- and HiNF-D related binding activities, whereas H3-ST519 and H1-FNC16 histone genes interact with the same CCAAT-box binding activity. These factors may function in regulatory coupling of the expression of different histone gene classes. We discuss these results within the context of established and putative protein/DNA interaction sites in mammalian histone genes. This model suggests that heterogeneous permutations of protein/DNA interaction elements, which involve both general and cell cycle regulated DNA binding proteins, may govern the cellular competency to express and coordinately control multiple distinct histone genes.
Rights and Permissions
Citation: J Cell Biochem. 1991 Dec;47(4):337-51. Link to article on publisher's site
DOI of Published Version
Journal of cellular biochemistry
Van Wijnen, Andre J.; Lian, Jane B.; Stein, Janet L.; and Stein, Gary S., "Protein/DNA interactions involving ATF/AP1-, CCAAT-, and HiNF-D-related factors in the human H3-ST519 histone promoter: cross-competition with transcription regulatory sites in cell cycle controlled H4 and H1 histone genes" (1991). GSBS Student Publications. 1288.