Differential dependence on target site tissue for gene gun and intramuscular DNA immunizations
Graduate School of Biomedical Sciences; Department of Pathology
Medical Subject Headings
Animals; *Antibody Formation; Biolistics; Cytotoxicity, Immunologic; Female; *Gene Transfer Techniques; *Immunity, Cellular; Injections, Intramuscular; Mice; Mice, Inbred BALB C; Muscles; Skin; T-Lymphocytes, Cytotoxic; Time Factors; Vaccines, DNA
Life Sciences | Medicine and Health Sciences
Surgical ablations revealed different dependencies on the injection site tissue for i.m. needle and epidermal gene gun DNA immunizations. Excision of an injected muscle bundle within 10 min of DNA inoculation did not affect the magnitude or longevity of Ag-specific Ab responses. By contrast, biopsy of the skin target site up to 24 h after gene gun bombardment completely abrogated the Ab response in the majority of mice. The form of a DNA-expressed Ag (membrane bound, secreted, or intracellular) did not affect the temporal requirements for the skin and muscle target sites. Skin cells, but not muscle cells, were also required for DNA-induced CTL. We conclude that transfected cells in gene gun-bombarded skin, but not needle-injected muscle, play a central role in DNA-initiated Ab and CTL responses.
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Citation: J Immunol. 1997 May 15;158(10):4529-32.
Journal of immunology (Baltimore, Md. : 1950)
Torres, Celia Aurora Tiglao; Iwasaki, Akiko; Barber, Brian H.; and Robinson, Harriet L., "Differential dependence on target site tissue for gene gun and intramuscular DNA immunizations" (1997). GSBS Student Publications. 1260.