GSBS Student Publications


Role of different lymphoid tissues in the initiation and maintenance of DNA-raised antibody responses to the influenza virus H1 glycoprotein

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences



Document Type


Medical Subject Headings

Animals; Antibodies, Viral; Biolistics; DNA, Viral; Hemagglutinin Glycoproteins, Influenza Virus; Humans; Immunoglobulin G; Influenza A virus; Influenza Vaccines; Influenza, Human; Lymphoid Tissue; Mice; Mice, Inbred BALB C; Time Factors; Vaccines, DNA


Life Sciences | Medicine and Health Sciences


Antibody responses in mice immunized by a single gene gun inoculation of plasmid expressing the influenza virus H1 hemagglutinin and in mice immunized by a sublethal H1 influenza virus infection have been compared. Both immunizations raised long-lived serum responses that were associated with the localization of antibody-secreting cells (ASC) to the bone marrow. However, the kinetics of these responses were 4 to 8 weeks slower in the DNA-immunized than in the infection-primed mice. Following a gene gun booster, the presence of ASC in the inguinal lymph nodes, but not in other lymph nodes, revealed gene gun responses being initiated in the nodes that drain the skin target site. Both pre- and postchallenge, the DNA-immunized mice had 5- to 10-times-lower levels of antibody and ASC than the infection-primed mice.

Rights and Permissions

Citation: J Virol. 1996 Dec;70(12):9074-8.

Related Resources

Link to article in PubMed

Journal Title

Journal of virology

PubMed ID