GSBS Student Publications

Title

The rde-1 gene, RNA interference, and transposon silencing in C. elegans

Publication Date

1999-10-27

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Program in Molecular Medicine

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Double-stranded (ds) RNA can induce sequence-specific inhibition of gene function in several organisms. However, both the mechanism and the physiological role of the interference process remain mysterious. In order to study the interference process, we have selected C. elegans mutants resistant to dsRNA-mediated interference (RNAi). Two loci, rde-1 and rde-4, are defined by mutants strongly resistant to RNAi but with no obvious defects in growth or development. We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates. Interestingly, several, but not all, RNAi-deficient strains exhibit mobilization of the endogenous transposons. We discuss implications for the mechanism of RNAi and the possibility that one natural function of RNAi is transposon silencing.

Source

Cell. 1999 Oct 15;99(2):123-32.

Journal/Book/Conference Title

Cell

Related Resources

Link to Article in PubMed

PubMed ID

10535731

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